1,102 research outputs found

    A flexible framework for articulating how student teachers learn teaching, as described by participants in a national review of initial teacher education in England

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    The aim of this study was to elucidate approaches to teaching student teachers how to learn teaching. It was based on a view of learning to teach as complex but that this should not prevent those in the field of initial teacher education from constructing and articulating their own professional knowledge of how they teach student teachers. Using secondary data, generated for a national review of initial teacher education in England, it drew on multiple perspectives from those in the field, to inform the development of a flexible framework for articulating how student teachers learn teaching. The study was positioned within the paradigm of post-positivism and aligned to a critical realist philosophy. To this end, it illuminates the social structures of and for professional practice, through qualitative thematic analysis, which took a hybrid approach to theme generation. The themes generated were used to develop the ‘Pillars of Interaction and Interconnecting Bridges Framework for Articulating ITE Practice’. Rather than focusing on an aspect of practice, the framework and themes that generated it, took a holistic view of initial teacher education, whilst still representing its complexity. Central to the framework and a key finding from the study is the importance of viewing student teachers as teachers of their own learning, learning to learn how to teach. Thus, the findings illuminate how to teach future teachers to also be future learners of teaching. The study offers teacher educators, as well as those developing initial teacher education strategy, a flexible framework to articulate, guide and further develop practice. As such, it contributes to initial teacher education discussion and debates informing current and future policy

    A comparative genomics approach to understanding the biosynthesis of the sunscreen scytonemin in cyanobacteria

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    BACKGROUND: The extracellular sunscreen scytonemin is the most common and widespread indole-alkaloid among cyanobacteria. Previous research using the cyanobacterium Nostoc punctiforme ATCC 29133 revealed a unique 18-gene cluster (NpR1276 to NpR1259 in the N. punctiforme genome) involved in the biosynthesis ofscytonemin. We provide further genomic characterization of these genes in N. punctiforme and extend it to homologous regions in other cyanobacteria. RESULTS: Six putative genes in the scytonemin gene cluster (NpR1276 to NpR1271 in the N. punctiforme genome), with no previously known protein function and annotated in this study as scyA to scyF, are likely involved in the assembly of scytonemin from central metabolites, based on genetic, biochemical, and sequence similarity evidence. Also in this cluster are redundant copies of genes encoding for aromatic amino acid biosynthetic enzymes. These can theoretically lead to tryptophan and the tyrosine precursor, p-hydroxyphenylpyruvate, (expected biosynthetic precursors of scytonemin) from end products of the shikimic acid pathway. Redundant copies of the genes coding for the key regulatory and rate-limiting enzymes of the shikimic acid pathway are found there as well. We identified four other cyanobacterial strains containing orthologues of all of these genes, three of them by database searches (Lyngbya PCC 8106, Anabaena PCC 7120, and Nodularia CCY 9414) and one by targeted sequencing (Chlorogloeopsis sp. strain Cgs-089; CCMEE 5094). Genomic comparisons revealed that mostscytonemin-related genes were highly conserved among strains and that two additional conserved clusters, NpF5232 to NpF5236 and a putative two-component regulatory system (NpF1278 and NpF1277), are likely involved in scytonemin biosynthesis and regulation, respectively, on the basis of conservation and location. Since many of the protein product sequences for the newly described genes, including ScyD, ScyE, and ScyF, have export signal domains, while others have putative transmembrane domains, it can be inferred that scytonemin biosynthesis is compartmentalized within the cell. Basic structural monomer synthesis and initial condensation are most likely cytoplasmic, while later reactions are predicted to be periplasmic. CONCLUSION: We show that scytonemin biosynthetic genes are highly conserved among evolutionarily diverse strains, likely include more genes than previously determined, and are predicted to involve compartmentalization of the biosynthetic pathway in the cell, an unusual trait for prokaryotes

    The consequences of tree disease and pre-emptive felling on functional and genetic connectivity for woodland invertebrates

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    Funding FAP was funded by the Macaulay Development Trust and the Woodland Trust. RJM and JAS were supported by the Scottish Government’s Rural and Environment Research and Analysis Directorate 2016-2021 strategic research programme. Development by SCFP of the landscape genetics capability in the customised version of RangeShifter was funded by programme Blanc of l’Agence nationale de la recherche. JMJT was supported by NERC grant NE/T006935/1 ‘Wildlife Corridors: Do they work and who benefits?’ Acknowledgments We thank the Macaulay Development Trust and the Woodland Trust for funding FAP, the Scottish Government’s Rural and Environment Research and Analysis Directorate for funding RJM and JAS, programme Blanc of l’Agence nationale de la recherche for funding SCFP, and NERC for funding JT. We thank the Woodland Trust for access to the National Canopy Map for England and WalesPeer reviewedPublisher PD
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